en پژوهشی اصيل Original Research <span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span cambria="" style="font-family:">Aims:</span></b>&nbsp;<i><span cambria="" style="font-family:">Teucrium polium</span></i><span cambria="" style="font-family:"> is a medicinal plant that is used due to its antispasmodic and antimicrobial properties. This study aimed to evaluate the effect of the essential oil of <i>T. polium</i> against fungal, bacterial strains, and <i>Giardia lamblia</i>. </span></span></span></span><br> <span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span cambria="" style="font-family:">Materials & Methods:</span></b><span cambria="" style="font-family:">&nbsp;In this clinical laboratory study, <i>Teucrium polium</i> was collected from the Dena mountain area in Yasuj, Iran.</span> <span cambria="" style="font-family:">Essential oil was prepared from plant powder.</span> <span cambria="" style="font-family:">Clinical Aspergillus species were isolated from otomycosis patients. For Candida species, the researchers used clinical isolates with otomycosis and vaginitis origin.</span> <span cambria="" style="font-family:">33 Aspergillus strains, 49 Candida species, and 33 non-albicans species were used as samples.</span> <span cambria="" style="font-family:">The samples were collected and cultured on Sabouraud dextrose agar and identified based on PCR-sequencing analysis. Different concentrations of essential oil were assessed by broth microdilution method against clinical fungi and bacterial isolates.</span> <span cambria="" style="font-family:">Also, anti-Giardia activity of this essential oil was investigated at different times and concentrations.</span> <span cambria="" style="font-family:">Chemical analysis of the essential oil was done by Gas Chromatography&ndash;Mass Spectrometry.</span></span></span></span><br> <span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span cambria="" style="font-family:">Findings:</span></b><span cambria="" style="font-family:">&nbsp;The inhibitory effect of essential oil of <i>T. polium</i> on Candida and Aspergillus strains was varied and dependent on species. Generally,</span><b> </b><span cambria="" style="font-family:">the effect of essential oil on non-albicans species was better than <i>C. albicans</i> isolates. Also, essential oil had an inhibitory effect on <i>E. coli</i> and <i>Klebsiella</i> but did not affect </span><span cambria="" style="font-family:">methicillin-resistant <i>Staphylococcus aureus</i></span><span cambria="" style="font-family:">. Its effect on Giardia isolates was dependent on time and concentration. Oxygenated monoterpenes are the major compound of <i>T. polium</i>.</span></span></span></span><br> <span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span cambria="" style="font-family:">Conclusion:</span></b><span cambria="" style="font-family:">&nbsp;<i>Teucrium polium</i> essential oil has a considerable inhibitory effect on different strains of microorganisms.</span></span></span></span><br> <span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span style="font-family:"Cambria","serif""></span></span></span></span><br> &nbsp; Teucrium polium,Candida,Aspergillus,Bacteria,Giardia 125 132 http://jccs.yums.ac.ir/browse.php?a_code=A-10-178-1&slc_lang=en&sid=1 Gh. Sabz 10031947532846005712 10031947532846005712 No Department of Otorhinolaryngology, School of Medicine, Yasuj University of Medical Sciences, Yasuj, Iran D. Razmjoue 10031947532846005713 10031947532846005713 No “Medicinal Plants Research Center” and “School of Medicine”, Yasuj University of Medical Sciences, Yasuj, Iran H. Sadeghi Mansourkhani 10031947532846005714 10031947532846005714 No “Medicinal Plants Research Center” and “Department of Pharmacology, School of Medicine”, Yasuj University of Medical Sciences, Yasuj, Iran M. Salahi 10031947532846005715 10031947532846005715 No “Medicinal Plants Research Center” and “School of Medicine”, Yasuj University of Medical Sciences, Yasuj, Iran S. Milani 10031947532846005716 10031947532846005716 No “Medicinal Plants Research Center” and “School of Medicine”, Yasuj University of Medical Sciences, Yasuj, Iran T. Ahmadian 10031947532846005717 10031947532846005717 No “Medicinal Plants Research Center” and “School of Medicine”, Yasuj University of Medical Sciences, Yasuj, Iran M. Gharaghani maralgharaghani@gmail.com 10031947532846005718 10031947532846005718 Yes “Medicinal Plants Research Center” and “School of Medicine”, Yasuj University of Medical Sciences, Yasuj, Iran S. Nouripour-Sisakht 10031947532846005719 10031947532846005719 No “Medicinal Plants Research Center” and “Department of Microbiology, School of Medicine”, Yasuj University of Medical Sciences, Yasuj, Iran