en پژوهشی اصيل Original Research <span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span cambria="" style="font-family:">Aims:</span></b><b> </b><span cambria="" style="font-family:">Because of the increasing prevalence of fungal infections and antifungal resistance, researchers are seeking new antifungal medications and alternatives. This study aimed to assess the antifungal and anti-biofilm propertie of <i>Myrtus communis </i>L. essential oil and effect of hydroalcoholic extract on <i>Candida</i> species isolated from clinical specimens.</span></span></span></span><br> <span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span cambria="" style="font-family:">Materials & Methods:</span></b><span cambria="" style="font-family:"> A total of 65 <i>Candida</i> species isolated from clinical samples were evaluated in this study. The chemical composition of the essential oil was analyzed by gas chromatography-mass spectrometry (GC-MS). Besides, the antifungal and biofilm activity of <i>M. communis </i>against <i>Candida</i> isolates was compared with that of fluconazole </span></span></span></span><br> <span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span cambria="" style="font-family:">Findings:</span></b> <span cambria="" lang="EN-GB" style="font-family:">A total of 22 compounds, displaying 99.88% of the <i>Myrtus Communis</i></span><span cambria="" style="font-family:"> leaves OE</span><span cambria="" lang="EN-GB" style="font-family:">, were identified and the major components were found to be &alpha;-pinene (51.22%), eucalyptol (16.88%), linalool (15.92%), and linalool acetate (4.03%). The main components of fruit EO were nonadecane (44.00%), heneicosane (19.60%), &alpha;-pinene (12.80%), and eucalyptol (10.08%). </span><span cambria="" style="font-family:">The minimum inhibitory concentration (MIC) of the hydroalcoholic extract of <i>M. communis</i> was lower against <i>C. parapsilosis</i> compared to <i>C. albicans </i>and <i>C. glabrata</i>. Biofilm formation in different <i>Candida </i>strains was inhibited at <i>M. communis</i> essential oil concentrations of 2.5-0.0156&micro;l/mL. </span></span></span></span><br> <span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span cambria="" style="font-family:">Conclusion: </span></b><i><span cambria="" style="font-family:">M. communis </span></i><span cambria="" style="font-family:">exhibited considerable antifungal effects against <i>Candida </i>species. Also, the inhibitory effect of <i>M. communis</i> essential oil against <i>C. albicans</i> biofilm formation was higher than that of fluconazole. </span></span></span></span><br> <span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span style="font-family:"Cambria","serif""></span></span></span></span><br> &nbsp; Myrtus communis L., Essential oil, Hydroalcoholic extract, Candida, Fluconazole, Biofilm 177 182 http://jccs.yums.ac.ir/browse.php?a_code=A-10-178-2&slc_lang=en&sid=1 S. Nouripour Sisakht 10031947532846004669 10031947532846004669 No Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, Iran D. Razmjoue 10031947532846004670 10031947532846004670 No Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, Iran H. Sadeghi Mansourkhani 10031947532846004671 10031947532846004671 No Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, Iran N. Hashemi 10031947532846004672 10031947532846004672 No Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, Iran F. kianfar 10031947532846004673 10031947532846004673 No Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, Iran M. Salahi 10031947532846004674 10031947532846004674 No Department of Microbiology, School of Medicine, Yasuj University of Medical Sciences, Yasuj, Iran A. Saadatnia 10031947532846004675 10031947532846004675 No Department of Microbiology, School of Medicine, Yasuj University of Medical Sciences, Yasuj, Iran M. Gharaghani 10031947532846004676 10031947532846004676 Yes Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, Iran