IMEMR
EMRO
66.92
Index Copernicus
ICICICICICICICIC
Volume 5, Issue 4 (2024)
J Clinic Care Skill 2024, 5(4): 233-239 |
Back to browse issues page
Article Type:
Subject:
History
Received: 2024/09/22 | Accepted: 2024/11/21 | Published: 2024/11/30
Received: 2024/09/22 | Accepted: 2024/11/21 | Published: 2024/11/30
How to cite this article
Zareifrar N, Sharifi A, Khosravani S, Zoladl M, Khoramrooz S, Sharifi K. Comparison of the Antibacterial Activity of Hydroalcoholic Extracts of Cydonia oblonga and Hypericum perforatum with Clindamycin. J Clinic Care Skill 2024; 5 (4) :233-239
URL: http://jccs.yums.ac.ir/article-1-302-en.html
URL: http://jccs.yums.ac.ir/article-1-302-en.html
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Rights and permissions
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
1- Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran
2- Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
3- Social Determinants of Health Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
4- Student Research Committee, Kazerun Branch, Islamic Azad University, Kazerun, Iran
2- Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
3- Social Determinants of Health Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
4- Student Research Committee, Kazerun Branch, Islamic Azad University, Kazerun, Iran
Abstract (339 Views)
Aims: The ability of Staphylococcus aureus for biofilm formation caused increased bacterial viability in the host environment and antibiotic resistance. The antibacterial effects of plants such as Cydonia oblonga and Hypericum perforatum were known; However synergic effect of them compared to clindamycin was not found in the previous studies. Therefore, the aim of this study was to compare the antibacterial activity of hydroalcoholic extracts of Cydonia oblonga, Hypericum perforatum and synergistic effect of them against clinical isolates of biofilm formation with Clindamycin.
Materials & Methods: In this descriptive and analytical study, 148 clinical strains were collected. Phenotypic analysis was performed by concord plate agar method and genotypic checking by PCR genes was performed simultaneously. MIC was obtained by microbroth dilution method. Data were analyzed by SPSS software version 21 and descriptive.
Findings: From 148 clinical isolates of Staphylococcus aureus, 95 isolates (64.2%) of Staphylococcus aureus forming biofilm were isolated by Congord Agar phenotypic method which 12 isolates had genes during PCR.icaA.icaD clfA and fnbA. There was a significant difference between groups in MIC, MBC, and ZOI (p=0.0001). However, Clindamycin and Hypericum perforatum hydroalcoholic extract had lower MIC, and MBC against S. aureus biofilm, respectively compared to other interventions.
Conclusion: The hydroalcoholic extract of Hypericum perforatum can be prescribed as a complementary medicine in the treatment of S. aureus biofilm, along with clindamycin.
Materials & Methods: In this descriptive and analytical study, 148 clinical strains were collected. Phenotypic analysis was performed by concord plate agar method and genotypic checking by PCR genes was performed simultaneously. MIC was obtained by microbroth dilution method. Data were analyzed by SPSS software version 21 and descriptive.
Findings: From 148 clinical isolates of Staphylococcus aureus, 95 isolates (64.2%) of Staphylococcus aureus forming biofilm were isolated by Congord Agar phenotypic method which 12 isolates had genes during PCR.icaA.icaD clfA and fnbA. There was a significant difference between groups in MIC, MBC, and ZOI (p=0.0001). However, Clindamycin and Hypericum perforatum hydroalcoholic extract had lower MIC, and MBC against S. aureus biofilm, respectively compared to other interventions.
Conclusion: The hydroalcoholic extract of Hypericum perforatum can be prescribed as a complementary medicine in the treatment of S. aureus biofilm, along with clindamycin.
Keywords:
References
1. Mouajou V, Adams K, DeLisle G, Quach C. Hand hygiene compliance in the prevention of hospital-acquired infections: A systematic review. J Hosp Infect. 2022;119:33-48. [Link] [DOI:10.1016/j.jhin.2021.09.016]
2. Aljamali NM, Al Najim MM. Review in hospital-acquired infection. Int J Adv Eng Res. 2020;20(III):7-20. [Link]
3. Brinkwirth S, Ayobami O, Eckmanns T, Markwart R. Hospital-acquired infections caused by enterococci: A systematic review and meta-analysis, WHO European Region, 1 January 2010 to 4 February 2020. Euro Surveill. 2021;26(45):2001628. [Link] [DOI:10.2807/1560-7917.ES.2021.26.45.2001628]
4. Massart N, Mansour A, Ross JT, Piau C, Verhoye JP, Tattevin P, et al. Mortality due to hospital-acquired infection after cardiac surgery. J Thorac Cardiovasc Surg. 2022;163(6):2131-40. [Link] [DOI:10.1016/j.jtcvs.2020.08.094]
5. Liu JY, Dickter JK. Nosocomial infections: A history of hospital-acquired infections. Gastrointest Endosc Clin N Am. 2020;30(4):637-52. [Link] [DOI:10.1016/j.giec.2020.06.001]
6. Patil RK, Kabera B, Muia CK, Ale BM. Hospital acquired infections in a private paediatric hospital in Kenya: A retrospective cross-sectional study. Pan Afr Med J. 2022;41:28. [Link] [DOI:10.11604/pamj.2022.41.28.25820]
7. Gandasasmita N, Li J, Loane DJ, Semple BD. Experimental models of hospital-acquired infections after traumatic brain injury: Challenges and opportunities. J Neurotrauma. 2024;41(7-8):752-70. [Link] [DOI:10.1089/neu.2023.0453]
8. Hu LQ, Wang J, Huang A, Wang D, Wang J. COVID-19 and improved prevention of hospital-acquired infection. Br J Anaesth. 2020;125(3):e318-9. [Link] [DOI:10.1016/j.bja.2020.05.037]
9. Abban MK, Ayerakwa EA, Mosi L, Isawumi A. The burden of hospital acquired infections and antimicrobial resistance. Heliyon. 2023;9(10):e20561. [Link] [DOI:10.1016/j.heliyon.2023.e20561]
10. Majlander J, Anttila VJ, Nurmi W, Seppälä A, Tiedje J, Muziasari W. Routine wastewater-based monitoring of antibiotic resistance in two Finnish hospitals: Focus on carbapenem resistance genes and genes associated with bacteria causing hospital-acquired infections. J Hosp Infect. 2021;117:157-64. [Link] [DOI:10.1016/j.jhin.2021.09.008]
11. Idrees M, Sawant S, Karodia N, Rahman A. Staphylococcus aureus Biofilm: Morphology, genetics, pathogenesis and treatment strategies. Int J Environ Res Public Health. 2021;18(14):7602. [Link] [DOI:10.3390/ijerph18147602]
12. Tuon FF, Suss PH, Telles JP, Dantas LR, Borges NH, Ribeiro VST. Antimicrobial treatment of staphylococcus aureus Biofilms. Antibiotics. 2023;12(1):87. [Link] [DOI:10.3390/antibiotics12010087]
13. Hair BB, Conley ME, Wienclaw TM, Conley MJ, Heaton MJ, Berges BK. Synergistic activity of silver nanoparticles and vancomycin against a spectrum of Staphylococcus aureus biofilm types. J Bacteriol Mycol. 2018;5(9):1089. [Link] [DOI:10.1101/337436]
14. Nourbakhsh F, Namvar AE. Detection of genes involved in biofilm formation in Staphylococcus aureus isolates. GMS Hyg Infect Control. 2016;11:Doc07. [Link]
15. Nandhini P, Kumar P, Mickymaray S, Alothaim AS, Somasundaram J, Rajan M. Recent developments in methicillin-resistant staphylococcus aureus (MRSA) treatment: A review. Antibiotics. 2022;11(5):606. [Link] [DOI:10.3390/antibiotics11050606]
16. Anpalagan K, Dotel R, MacFadden DR, Smith S, Voss L, Petersiel N, et al. Does adjunctive clindamycin have a role in Staphylococcus aureus bacteremia? A protocol for the adjunctive treatment domain of the Staphylococcus aureus Network Adaptive Platform (SNAP) randomized controlled trial. Clin Infect Dis. 2024;79(3):626-34. [Link] [DOI:10.1093/cid/ciae289]
17. Armengol Álvarez L, Van De Sijpe G, Desmet S, Metsemakers WJ, Spriet I, Allegaert K, et al. Ways to improve insights into clindamycin pharmacology and pharmacokinetics tailored to practice. Antibiotics. 2022;11(5):701. [Link] [DOI:10.3390/antibiotics11050701]
18. Thapa D, Pyakurel S, Thapa S, Lamsal S, Chaudhari M, Adhikari N, et al. Staphylococcus aureus with inducible clindamycin resistance and methicillin resistance in a tertiary hospital in Nepal. Trop Med Health. 2021;49(1):99. [Link] [DOI:10.1186/s41182-021-00392-2]
19. Moghadam Fard A, Nikbakht T, Babaei N, Pouyamanesh M, Afzalian A, Kharazmkia A, et al. Role of medicinal plants in treatment of inflammatory diseases. Kindle. 2022;2(1):1-139. [Link]
20. Ahluwalia O, Singh PC, Bhatia R. A review on drought stress in plants: Implications, mitigation and the role of plant growth promoting rhizobacteria. Resour Environ Sustain. 2021;5:100032. [Link] [DOI:10.1016/j.resenv.2021.100032]
21. Alesiani D, Canini A, D'Abrosca B, DellaGreca M, Fiorentino A, Mastellone C, et al. Antioxidant and antiproliferative activities of phytochemicals from Quince (Cydonia vulgaris) peels. Food Chem. 2010;118(2):199-207. [Link] [DOI:10.1016/j.foodchem.2009.04.098]
22. Al-Snafi AE. The medical importance of Cydonia oblonga-A review. IOSR J Pharm. 2016;6(6):87-99. [Link]
23. Al-Khazraji SK. Phytochemical screening and antibacterial activity of the crude extract of Cydonia oblonga seeds. Glob Adv Res J Microbiol. 2013;2(8):137-40. [Link] [DOI:10.12816/0010090]
24. Southwell IA, Bourke CA. Seasonal variation in hypericin content of Hypericum perforatum L. (St. John's Wort). Phytochemistry. 2001;56(5):437-41. [Link] [DOI:10.1016/S0031-9422(00)00411-8]
25. Nazlı O, Baygar T, Demirci Dönmez ÇE, Dere Ö, Uysal Aİ, Aksözek A, et al. Antimicrobial and antibiofilm activity of polyurethane/Hypericum perforatum extract (PHPE) composite. Bioorg Chem. 2019;82:224-8. [Link] [DOI:10.1016/j.bioorg.2018.08.017]
26. Barnes J, Anderson LA, Phillipson JD. St John's wort (Hypericum perforatum L.): A review of its chemistry, pharmacology and clinical properties. J Pharm Pharmacol. 2001;53(5):583-600. [Link] [DOI:10.1211/0022357011775910]
27. Saddiqe Z, Naeem I, Maimoona A. A review of the antibacterial activity of Hypericum perforatum L. J Ethnopharmacol. 2010;131(3):511-21. [Link] [DOI:10.1016/j.jep.2010.07.034]
28. Akhbari M, Ebrahimian M. Comparison of hypericin content, antioxidant, antimicrobial and cytotoxic activities of Hypericum perforatum L. from three geographic regions of Iran. J Gorgan Univ Med Sci. 2017;19(1):89-95. [Persian] [Link]
29. Sadeghi H, Hosseinzadeh S, Akbartabar Touri M, Ghavamzadeh M, Jafari Barmak M, Sayahi M, et al. Hepatoprotective effect of Rosa canina fruit extract against carbon tetrachloride induced hepatotoxicity in rat. Avicenna J Phytomed. 2016;6(2):181-8. [Link]
30. Abbaszadegan A, Gholami A, Ghahramani Y, Ghareghan R, Ghareghan M, Kazemi A, et al. Antimicrobial and cytotoxic activity of Cuminum cyminum as an intracanal medicament compared to chlorhexidine gel. Iran Endod J. 2016;11(1):44-50. [Link] [DOI:10.5395/rde.2015.40.1.50]
31. Koohsari H, Ghaemi EA, Sadegh Sheshpoli M, Jahedi M, Zahiri M. The investigation of antibacterial activity of selected native plants from North of Iran. J Med Life. 2015;8(Spec Iss 2):38-42. [Link]
32. Rothenburger S, Spangler D, Bhende S, Burkley D. In vitro antimicrobial evaluation of Coated VICRYL* Plus Antibacterial Suture (coated polyglactin 910 with triclosan) using zone of inhibition assays. Surg Infect. 2002;3(Suppl 1):S79-87. [Link] [DOI:10.1089/sur.2002.3.s1-79]
33. Tille P. Bailey & Scott's diagnostic microbiology. London: Elsevier Health Sciences; 2015. [Link]
34. Demir C, Demirci M, Yigin A, Tokman HB, Cetik Yildiz S. Presence of biofilm and adhesin genes in Staphylococcus aureus strains taken from chronic wound infections and their genotypic and phenotypic antimicrobial sensitivity patterns. Photodiagnosis Photodyn Ther. 2020;29:101584. [Link] [DOI:10.1016/j.pdpdt.2019.101584]
35. Yazdani R, Oshaghi M, Havayi A, Pishva E, Salehi R, Sadeghizadeh M, et al. Detection of icaAD gene and biofilm formation in Staphylococcus aureus isolates from wound infections. Iran J Public Health. 2006;35(2):25-8. [Link]
36. Dastgheyb S, Parvizi J, Shapiro IM, Hickok NJ, Otto M. Effect of biofilms on recalcitrance of staphylococcal joint infection to antibiotic treatment. J Infect Dis. 2015;211(4):641-50. [Link] [DOI:10.1093/infdis/jiu514]
37. Wang L, Yu F, Yang L, Li Q, Zhang X, Zeng Y, et al. Prevalence of virulence genes and biofilm formation among Staphylococcus aureus clinical isolates associated with lower respiratory infection. Afr J Microbiol Res. 2010;4(23):2566-9. [Link]
38. Coutinho Vde L, Paiva RM, Reiter KC, De-Paris F, Barth AL, Machado AB. Distribution of erm genes and low prevalence of inducible resistance to clindamycin among staphylococci isolates. Braz J Infect Dis. 2010;14(6):564-8. [Link] [DOI:10.1590/S1413-86702010000600004]
39. Mollaei M, Rashki A. The prevalence of adhesive surface encoding genes in staphylococcus aureus isolated from hospitalized patients in Zabol-Iran by multiplex PCR. J Adv Biomed Sci. 2016;6(3):296-302. [Persian] [Link]
40. Aygül A, Şerbetçi T. The antibacterial and antivirulent potential of Hypericum lydium against Staphylococcus aureus: Inhibition of growth, biofilm formation, and hemolytic activity. Eur J Integr Med. 2020;35:101061. [Link] [DOI:10.1016/j.eujim.2020.101061]
41. Bahmani M, Taherikalani M, Khaksarian M, Rafieian-Kopaei M, Ashrafi B, Nazer M, et al. The synergistic effect of hydroalcoholic extracts of Origanum vulgare, Hypericum perforatum and their active components carvacrol and hypericin against Staphylococcus aureus. Future Sci OA. 2019;5(3):FSO371. [Link] [DOI:10.4155/fsoa-2018-0096]