Showing 4 results for Gharaghani
Gh. Sabz, D. Razmjoue, H. Sadeghi Mansourkhani , M. Salahi, S. Milani, T. Ahmadian, M. Gharaghani , S. Nouripour-Sisakht ,
Volume 3, Issue 3 (Summer 2022)
Abstract
Aims: Teucrium polium is a medicinal plant that is used due to its antispasmodic and antimicrobial properties. This study aimed to evaluate the effect of the essential oil of T. polium against fungal, bacterial strains, and Giardia lamblia.
Materials & Methods: In this clinical laboratory study, Teucrium polium was collected from the Dena mountain area in Yasuj, Iran. Essential oil was prepared from plant powder. Clinical Aspergillus species were isolated from otomycosis patients. For Candida species, the researchers used clinical isolates with otomycosis and vaginitis origin. 33 Aspergillus strains, 49 Candida species, and 33 non-albicans species were used as samples. The samples were collected and cultured on Sabouraud dextrose agar and identified based on PCR-sequencing analysis. Different concentrations of essential oil were assessed by broth microdilution method against clinical fungi and bacterial isolates. Also, anti-Giardia activity of this essential oil was investigated at different times and concentrations. Chemical analysis of the essential oil was done by Gas Chromatography–Mass Spectrometry.
Findings: The inhibitory effect of essential oil of T. polium on Candida and Aspergillus strains was varied and dependent on species. Generally, the effect of essential oil on non-albicans species was better than C. albicans isolates. Also, essential oil had an inhibitory effect on E. coli and Klebsiella but did not affect methicillin-resistant Staphylococcus aureus. Its effect on Giardia isolates was dependent on time and concentration. Oxygenated monoterpenes are the major compound of T. polium.
Conclusion: Teucrium polium essential oil has a considerable inhibitory effect on different strains of microorganisms.
S. Nouripour Sisakht, D. Razmjoue, H. Sadeghi Mansourkhani, N. Hashemi, F. Kianfar, M. Salahi, A. Saadatnia, M. Gharaghani,
Volume 3, Issue 4 (Fall 2022)
Abstract
Aims: Because of the increasing prevalence of fungal infections and antifungal resistance, researchers are seeking new antifungal medications and alternatives. This study aimed to assess the antifungal and anti-biofilm propertie of Myrtus communis L. essential oil and effect of hydroalcoholic extract on Candida species isolated from clinical specimens.
Materials & Methods: A total of 65 Candida species isolated from clinical samples were evaluated in this study. The chemical composition of the essential oil was analyzed by gas chromatography-mass spectrometry (GC-MS). Besides, the antifungal and biofilm activity of M. communis against Candida isolates was compared with that of fluconazole
Findings: A total of 22 compounds, displaying 99.88% of the Myrtus Communis leaves OE, were identified and the major components were found to be α-pinene (51.22%), eucalyptol (16.88%), linalool (15.92%), and linalool acetate (4.03%). The main components of fruit EO were nonadecane (44.00%), heneicosane (19.60%), α-pinene (12.80%), and eucalyptol (10.08%). The minimum inhibitory concentration (MIC) of the hydroalcoholic extract of M. communis was lower against C. parapsilosis compared to C. albicans and C. glabrata. Biofilm formation in different Candida strains was inhibited at M. communis essential oil concentrations of 2.5-0.0156µl/mL.
Conclusion: M. communis exhibited considerable antifungal effects against Candida species. Also, the inhibitory effect of M. communis essential oil against C. albicans biofilm formation was higher than that of fluconazole.
M. Gharaghani, Z. Ashrafzade, D. Razmjoue, H. Sadeghi Mansourkhani, M. Salahi, H. Yousefi Mehryan, T. Talaie, S. Nouripour-Sisakht,
Volume 4, Issue 1 (Winter 2023)
Abstract
S. Nouripour-Sisakht, A. Diba, D. Razmjoue, H. Sadeghi Mansourkhani , P. Zanganeh , M. Salahi, M. Gharaghani ,
Volume 5, Issue 1 (Winter 2024)
Abstract
Aims: Candidiasis is the most opportunistic infection with a high rate of recurrent infection. Salvia macrosiphon has an antibacterial effect; however, its antifungal effect was not studied. This study aimed to investigate the chemical composition of essential oil from leaves of Salvia macrosiphon and its antifungal activity compared with fluconazole.
Materials & Methods: Salvia macrosiphon leaves, a native plant of Kogiluyeh and Boyerahmad province, were collected from Zagros Heights and used in this study. Then, the essential oil of this plant was tested for antibacterial and antifungal properties and compared with fluconazole. The chemical composition of the essential oil was analyzed by GC/MS (Gas Chromatography Mass Spectrometry), and the antifungal activity of the plant's essential oil was compared with that of fluconazole.
Findings: The results of GC-MS analysis proved the presence of at least 29 compounds in the essential oil of Salvia macrosiphon. Amon these constitute butyl benzoate (49.16%), n-hexyl benzoate (7%), and isopatolenol (4.83%) were the main compounds. The minimum inhibitory concentration (MIC) of the essential oil (µl/ml) of Salvia macrosiphon and fluconazole (μg/ml) were 0.44 and 0.7 for Candida albicans, 0.056 and 0.7 for C. glabrata, and 0.1 and 0.088 for C. parapsilosis, respectively. Also, statistical analysis demonstrated a significant difference between the mean of fluconazole and essential oil in total Candida isolates (p=0.001).
Conclusion: The essential oil of Salvia macrosiphon has stronger antifungal activity than fluconazole.
