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Volume 5, Issue 3 (2024)
J Clinic Care Skill 2024, 5(3): 157-163 |
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Ethics code: IR. YUMS.REC.1400.201
History
Received: 2024/05/26 | Accepted: 2024/09/22 | Published: 2024/10/6
Received: 2024/05/26 | Accepted: 2024/09/22 | Published: 2024/10/6
How to cite this article
Masnavi E, Hassanzadeh S, Karimi K, Malekzadeh J, Khoramrooz S. Antibacterial Activities of Hydroalcoholic Extract of Rosa Canina L against Hospital Acquired Infections. J Clinic Care Skill 2024; 5 (3) :157-163
URL: http://jccs.yums.ac.ir/article-1-264-en.html
URL: http://jccs.yums.ac.ir/article-1-264-en.html
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1- Department of Obstetrics and Gynecology, School of Medicine, Yasuj University of Medical Sciences, Yasuj, Iran
2- Department of Internal Medicine, School of Medicine, Yasuj University of Medical Sciences, Yasuj, Iran
3- Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran
4- Department of Nutrition Sciences, School of Medicine, Yasuj University of Medical Sciences, Yasuj, Iran
5- Department of Microbiology, School of Medicine, Yasuj University of Medical Sciences, Yasuj, Iran
2- Department of Internal Medicine, School of Medicine, Yasuj University of Medical Sciences, Yasuj, Iran
3- Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran
4- Department of Nutrition Sciences, School of Medicine, Yasuj University of Medical Sciences, Yasuj, Iran
5- Department of Microbiology, School of Medicine, Yasuj University of Medical Sciences, Yasuj, Iran
Abstract (1069 Views)
Aims: Every year around the world, hospital infections and resistance to antibiotics lead to many complications in hospitalized patients. This study aimed to determine and compare the antibacterial effect of hydroalcoholic extract of Rosa canina plant (Rosa canina L) on Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Staphylococcus aureus bacteria isolated from surgical wounds in Yasuj hospitals.
Materials & Methods: In this cross-sectional study, the effect of Rosa canina L hydroalcoholic extract on 20 samples of Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae and Staphylococcus aureus was investigated. The Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC) and zone of inhibition (ZOI) of each clinical sample were investigated and compared with the standard sample. The results were statistically analyzed by SPSS 16 software.
Findings: Rosa canina L hydroalcoholic extract had no antibacterial effect on Pseudomonas aeruginosa. The lowest inhibitory concentration was related to Staphylococcus aureus 125 µg/ml, Escherichia coli 325 µg/ml and Klebsiella pneumoniae 350 µg/ml, respectively. The difference in minimum MIC, MBC and ZOI between clinical and standard samples was not statistically significant. The ZOI diameter of Klebsiella pneumoniae and Escherichia coli clinical samples were equal (9.8 mm).
Conclusion: The hydroalcoholic extract of Rosa canina L has a better antimicrobial effect on Staphylococcus aureus than the other 3 investigated bacteria and has no effect on Pseudomonas aeruginosa. There is no difference between the effect of the examined extract on clinical and standard samples, and the effect of this extract on different bacteria is different.
Materials & Methods: In this cross-sectional study, the effect of Rosa canina L hydroalcoholic extract on 20 samples of Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae and Staphylococcus aureus was investigated. The Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC) and zone of inhibition (ZOI) of each clinical sample were investigated and compared with the standard sample. The results were statistically analyzed by SPSS 16 software.
Findings: Rosa canina L hydroalcoholic extract had no antibacterial effect on Pseudomonas aeruginosa. The lowest inhibitory concentration was related to Staphylococcus aureus 125 µg/ml, Escherichia coli 325 µg/ml and Klebsiella pneumoniae 350 µg/ml, respectively. The difference in minimum MIC, MBC and ZOI between clinical and standard samples was not statistically significant. The ZOI diameter of Klebsiella pneumoniae and Escherichia coli clinical samples were equal (9.8 mm).
Conclusion: The hydroalcoholic extract of Rosa canina L has a better antimicrobial effect on Staphylococcus aureus than the other 3 investigated bacteria and has no effect on Pseudomonas aeruginosa. There is no difference between the effect of the examined extract on clinical and standard samples, and the effect of this extract on different bacteria is different.
Keywords:
References
1. Misha G, Chelkeba L, Melaku T. Bacterial profile and antimicrobial susceptibility patterns of isolates among patients diagnosed with surgical site infection at a tertiary teaching hospital in Ethiopia: A prospective cohort study. Ann Clin Microbiol Antimicrob. 2021;20(1):33. [Link] [DOI:10.1186/s12941-021-00440-z]
2. Orhan DD, Hartevioğlu A, Küpeli E, Yesilada E. In vivo anti-inflammatory and antinociceptive activity of the crude extract and fractions from Rosa canina L. fruits. J Ethnopharmacol. 2007;112(2):394-400. [Link] [DOI:10.1016/j.jep.2007.03.029]
3. Farsi S, Salama I, Escalante-Alderete E, Cervantes J. Multidrug-resistant enterococcal infection in surgical patients, what surgeons need to know. Microorganisms. 2023;11(2):238. [Link] [DOI:10.3390/microorganisms11020238]
4. Bereket W, Hemalatha K, Getenet B, Wondwossen T, Solomon A, Zeynudin A, et al. Update on bacterial nosocomial infections. Eur Rev Med Pharmacol Sci. 2012;16(8):1039-44. [Link]
5. Mauermann WJ, Nemergut EC. The anesthesiologist's role in the prevention of surgical site infections. Anesthesiology. 2006;105(2):413-21. [Link] [DOI:10.1097/00000542-200608000-00025]
6. Agnihotri N, Gupta V, Joshi R. Aerobic bacterial isolates from burn wound infections and their antibiograms-a five-year study. Burns. 2004;30(3):241-3. [Link] [DOI:10.1016/j.burns.2003.11.010]
7. Guggenheim M, Zbinden R, Handschin AE, Gohritz A, Altintas MA, Giovanoli P. Changes in bacterial isolates from burn wounds and their antibiograms: A 20-year study (1986-2005). Burns. 2009;35(4):553-60. [Link] [DOI:10.1016/j.burns.2008.09.004]
8. Bramardipa AAB, Bramardipa B, Dewa I, Sukrama M. Bacterial pattern and its susceptibility toward antibiotic on burn infection in Burn Unit Sanglah General Hospital. Bali Med J. 2019;8(1):328-33. [Link] [DOI:10.15562/bmj.v8i1.1456]
9. Nigussie D, Makonnen E, Legesse BA, Fekadu A, Davey G. Antimicrobial susceptibility of bacteria isolated from the infected wounds of patients with lymphoedema in East Wollega, Ethiopia. Trans R Soc Trop Med Hyg. 2020;114(12):962-73. [Link] [DOI:10.1093/trstmh/traa143]
10. Hailu D, Derbie A, Mekonnen D, Zenebe Y, Adem Y, Worku S, et al. Drug resistance patterns of bacterial isolates from infected wounds at Bahir Dar regional Health Research Laboratory center, Northwest Ethiopia. Ethiop J Health Dev. 2016;30(3):112-7. [Link]
11. Nobel FA, Islam S, Babu G, Akter S, Jebin RA, Sarker TC, et al. Isolation of multidrug resistance bacteria from the patients with wound infection and their antibiotics susceptibility patterns: A cross-sectional study. Ann Med Surg. 2022;84:104895. [Link] [DOI:10.1016/j.amsu.2022.104895]
12. Anand U, Jacobo-Herrera N, Altemimi A, Lakhssassi N. A comprehensive review on medicinal plants as antimicrobial therapeutics: Potential avenues of biocompatible drug discovery. Metabolites. 2019;9(11):258. [Link] [DOI:10.3390/metabo9110258]
13. De Kraker ME, Stewardson AJ, Harbarth S. Will 10 million people die a year due to antimicrobial resistance by 2050?. PLoS Med. 2016;13(11):e1002184. [Link] [DOI:10.1371/journal.pmed.1002184]
14. Chavan SS, Damale MG, Shinde DB, Sangshetti JN. Antibacterial and antifungal drugs from natural source: A review of clinical development. Nat Prod Clin Trials. 2018;1:114-64. [Link] [DOI:10.2174/9781681082134118010006]
15. Chin YW, Balunas MJ, Chai HB, Kinghorn AD. Drug discovery from natural sources. AAPS J. 2006;8(2):E239-53. [Link] [DOI:10.1007/BF02854894]
16. Boy HIA, Rutilla AJH, Santos KA, Ty AMT, Alicia IY, Mahboob T, et al. Recommended medicinal plants as source of natural products: A review. Digit Chin Med. 2018;1(2):131-42. [Link] [DOI:10.1016/S2589-3777(19)30018-7]
17. Mawalagedera SM, Callahan DL, Gaskett AC, Rønsted N, Symonds MR. Combining evolutionary inference and metabolomics to identify plants with medicinal potential. Front Ecol Evol. 2019;7:267. [Link] [DOI:10.3389/fevo.2019.00267]
18. Selahvarzian A, Alizadeh A, Baharvand PA, Eldahshan OA, Rasoulian B. Medicinal properties of Rosa canina L. Herb Med J. 2018;3(2):77-84. [Link]
19. Polumackanycz M, Kaszuba M, Konopacka A, Marzec-Wróblewska U, Wesolowski M, Waleron K, et al. Phenolic composition and biological properties of wild and commercial dog rose fruits and leaves. Molecules. 2020;25(22):5272. [Link] [DOI:10.3390/molecules25225272]
20. Fayaz F, Singh K, Gairola S, Ahmed Z, Shah BA. A comprehensive review on phytochemistry and pharmacology of Rosa species (Rosaceae). Curr Top Med Chem. 2024;24(4):364-78. [Link] [DOI:10.2174/0115680266274385231023075011]
21. Tolekova S, Sharmanov T, Sinyavskıy Y, Berzhanova R, Mammadov R, Aksoy ÖK, et al. Antioxidant, pharmacological, medical properties and chemical content of Rosa L. extracts. Int J Second Metab. 2020;7(3):200-12. [Link] [DOI:10.21448/ijsm.726140]
22. Cendrowski A, Kraśniewska K, Przybył JL, Zielińska A, Kalisz S. Antibacterial and antioxidant activity of extracts from rose fruits (Rosa rugosa). Molecules. 2020;25(6):1365. [Link] [DOI:10.3390/molecules25061365]
23. Iancu P, Soare R, Dinu M, Soare M, Bonea D, Popescu M. Analysis of the existing research regarding the use of the species Rosa canina L. Sci Pap Ser B Hortic. 2020;64(2). [Link]
24. Miklášová K, Petrová J, Terentjeva M, Cerna J, Kačániová M. Antimicrobial activity of Rosa canina flowers against selected microorganisms. J Microbiol Biotechnol Food Sci. 2015;4(Spec Iss 1):62-4. [Link] [DOI:10.15414/jmbfs.2015.4.special1.62-64]
25. Miljković VM, Nikolić L, Mrmošanin J, Gajić I, Mihajilov-Krstev T, Zvezdanović J, et al. Chemical profile and antioxidant and antimicrobial activity of Rosa canina L. dried fruit commercially available in Serbia. Int J Mol Sci. 2024;25(5):2518. [Link] [DOI:10.3390/ijms25052518]
26. Yilmaz SO, Ercisli S. Antibacterial and antioxidant activity of fruits of some rose species from Turkey. Rom Biotechnol Lett. 2011;16(4):6407-11. [Link]
27. WHO. Report on the burden of endemic health care-associated infection worldwide. Geneva: World Health Organization; 2011. [Link]
28. Sadeghi H, Hosseinzadeh S, Touri MA, Ghavamzadeh M, Barmak MJ, Sayahi M, et al. Hepatoprotective effect of Rosa canina fruit extract against carbon tetrachloride induced hepatotoxicity in rat. Avicenna J Phytomed. 2016;6(2):181-8. [Link]
29. Mansourian M, Mirzaei A, Azarmehr N, Vakilpour H, Kokhdan EP, Doustimotlagh AH. Hepatoprotective and antioxidant activity of hydroalcoholic extract of Stachys pilifera. Benth on acetaminophen-induced liver toxicity in male rats. Heliyon. 2019;5(12):e03029. [Link] [DOI:10.1016/j.heliyon.2019.e03029]
30. Abbaszadegan A, Gholami A, Ghahramani Y, Ghareghan R, Ghareghan M, Kazemi A, et al. Antimicrobial and cytotoxic activity of cuminum cyminum as an intracanal medicament compared to chlorhexidine gel. Iran Endod J. 2016;11(1):44-50. [Link] [DOI:10.5395/rde.2015.40.1.50]
31. Koohsari H, Ghaemi E, Sheshpoli MS, Jahedi M, Zahiri M. The investigation of antibacterial activity of selected native plants from North of Iran. J Med Life. 2015;8(Spec Iss 2):38-42. [Link]
32. Rothenburger S, Spangler D, Bhende S, Burkley D. In vitro antimicrobial evaluation of Coated VICRYL* Plus Antibacterial Suture (coated polyglactin 910 with triclosan) using zone of inhibition assays. Surg Infect. 2002;3 Suppl 1:S79-87. [Link] [DOI:10.1089/sur.2002.3.s1-79]
33. Mohammadi M, Akkafi H, Yazdanshenas S, Adibnejad M. Plasmid curing activity of four plant extracts on multiple resistant plasmid bearing klebsiella pnumoniae strains. J Med Plants. 2012;11(44):109-19. [Persian] [Link]
34. Yazdanpanah S, Shirvani F. Antimicrobial and antioxidant effects of ethanolic extract of rosa canina fruit on increasing the shelf life of chicken meat kept at rrefrigerator temperature. J Food Microbiol. 2021;8(1):88-101. [Persian] [Link]
35. Moustafa EM, Araby E, Elbahkery AL. Assessing the antimicrobial, antioxidant and anti-inflammatory potential of ethanolic extract of irradiated Rosa canina L. fruits. Egypt J Radiat Sci Appl. 2021;34(1):27-43. [Link]
36. Hacioglu M, Dosler S, Tan ASB, Otuk G. Antimicrobial activities of widely consumed herbal teas, alone or in combination with antibiotics: An in vitro study. PeerJ. 2017;5:e3467. [Link] [DOI:10.7717/peerj.3467]