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Volume 5, Issue 1 (2024)                   J Clinic Care Skill 2024, 5(1): 5-9 | Back to browse issues page
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Nouripour-Sisakht S, Diba A, Razmjoue D, Sadeghi Mansourkhani H, Zanganeh P, Salahi M et al . Comparing the Anticandidal Activities of Salvia macrosiphon essential oil and Fluconazole. J Clinic Care Skill 2024; 5 (1) :5-9
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1- Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
* Corresponding Author Address: Faculty of Medicine, Medicinal Plants Research Center, Yasuj University of Medical Sciences, Shahid Gurban Ali Jalil Street, Yasuj, Iran. Postal Code: 7591994799 (maralgharaghani@gmail.com)
Abstract   (1132 Views)
Aims: Candidiasis is the most opportunistic infection with a high rate of recurrent infection. Salvia macrosiphon has an antibacterial effect; however, its antifungal effect was not studied. This study aimed to investigate the chemical composition of essential oil from leaves of Salvia macrosiphon and its antifungal activity compared with fluconazole.
Materials & Methods: Salvia macrosiphon leaves, a native plant of Kogiluyeh and Boyerahmad province, were collected from Zagros Heights and used in this study. Then, the essential oil of this plant was tested for antibacterial and antifungal properties and compared with fluconazole.  The chemical composition of the essential oil was analyzed by GC/MS (Gas Chromatography Mass Spectrometry), and the antifungal activity of the plant's essential oil was compared with that of fluconazole.
Findings: The results of GC-MS analysis proved the presence of at least 29 compounds in the essential oil of Salvia macrosiphon. Amon these constitute butyl benzoate (49.16%), n-hexyl benzoate (7%), and isopatolenol (4.83%) were the main compounds. The minimum inhibitory concentration (MIC) of the essential oil (µl/ml) of Salvia macrosiphon and fluconazole (μg/ml) were 0.44 and 0.7 for Candida albicans, 0.056 and 0.7 for C. glabrata, and 0.1 and 0.088 for C. parapsilosis, respectively. Also, statistical analysis demonstrated a significant difference between the mean of fluconazole and essential oil in total Candida isolates (p=0.001).
Conclusion: The essential oil of Salvia macrosiphon has stronger antifungal activity than fluconazole.
 
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References
1. Gharaghani M, Taghipour S, Halvaeezadeh M, Mahmoudabadi AZ. Candiduria; a review article with specific data from Iran. Turkish J Urol. 2018;44(6):445. [Link] [DOI:10.5152/tud.2018.54069]
2. Ciurea CN, Kosovski I-B, Mare AD, Toma F, Pintea-Simon IA, Man A. Candida and candidiasis-opportunism versus pathogenicity: A review of the virulence traits. Microorganisms. 2020;8(6):857. [Link] [DOI:10.3390/microorganisms8060857]
3. Vila T, Sultan AS, Montelongo-Jauregui D, Jabra-Rizk MA. Oral candidiasis: a disease of opportunity. J Fungi. 2020;6(1):15. [Link] [DOI:10.3390/jof6010015]
4. Ordaya EE, Clement J, Vergidis P. The role of novel antifungals in the management of Candidiasis: A clinical perspective. Mycopathologia. 2023:188(6):937-48. [Link] [DOI:10.1007/s11046-023-00759-5]
5. Houšť J, Spížek J, Havlíček V. Antifungal drugs. Metabolites. 2020;10(3):106. [Link] [DOI:10.3390/metabo10030106]
6. Berkow EL, Lockhart SR. Fluconazole resistance in Candida species: A current perspective. Infection Drug Resist. 2017;10:237-45. [Link] [DOI:10.2147/IDR.S118892]
7. Maliki I, Moussaoui AE, Ramdani M, ELBadaoui K. Phytochemical screening and the antioxidant, antibacterial and antifungal activities of aqueous extracts from the leaves of Salvia officinalis planted in Morocco. Moroccan J Chem. 2021;9(2):354-68. [Link]
8. Farhadi F, Sahebkar A, Eghbali S. Chemical composition and biological activity of the essential oils of three Salvia species from South Khorasan: Salvia macrosiphon Boiss., Salvia spinosa L., and Salvia sharifii Rech. f. & Esfand. J Essent Oil Bearing Plant. 2023;26(3):695-704. [Link] [DOI:10.1080/0972060X.2023.2234418]
9. Balaei-Kahnamoei M, Eftekhari M, Ardekani MRS, Akbarzadeh T, Saeedi M, Jamalifar H, et al. Phytochemical constituents and biological activities of Salvia macrosiphon Boiss. BMC Chem. 2021;15(1):4. [Link] [DOI:10.1186/s13065-020-00728-9]
10. Banan ZM, Yaghobfar A, Mojab F. The chemical composition of Salvia macrosiphon seed: The chemical composition of Salvia macrosiphon seed. Iran J Pharmaceutical Sci. 2023;19(2):166-75. [Link]
11. Ashley ED. Antifungal drugs: special problems treating central nervous system infections. J Fungi. 2019;5(4):97. [Link] [DOI:10.3390/jof5040097]
12. Altinbaş R, Bariş A, Şen S, Öztürk R, Kiraz N. Comparison of the Sensititre YeastOne antifungal method with the CLSI M27-A3reference method to determine the activity of antifungal agents against clinical isolates of Candida spp. Turk J Med Sci. 2020;50(8):2024-31. [Link] [DOI:10.3906/sag-1909-97]
13. Neal CM, Martens MG. Clinical challenges in diagnosis and treatment of recurrent vulvovaginal candidiasis. SAGE Open Med. 2022;10:20503121221115201. [Link] [DOI:10.1177/20503121221115201]
14. Lee Y, Puumala E, Robbins N, Cowen LE. Antifungal drug resistance: molecular mechanisms in Candida albicans and beyond. Chem Rev. 2020;121(6):3390-411. [Link] [DOI:10.1021/acs.chemrev.0c00199]
15. Subramaniyan V, Kayarohanam S, Kumar JA, Kumarasamy V. Impact of herbal drugs and its clinical application. Int J Res Pharmaceutical Sci. 2019;10(2):1340-5. [Link] [DOI:10.26452/ijrps.v10i2.537]
16. Sajadi S, Emami Sa, Nemati R. Composition of the essential oil of Salvia macrosiphon boiss. Pharmaceutical Sci. 2000;(3):51-6. [Persian] [Link]
17. Taarit MB, Msaada K, Hosni K, Hammami M, Kchouk ME, Marzouk B. Plant growth, essential oil yield and composition of sage (Salvia officinalis L.) fruits cultivated under salt stress conditions. Industrial Crops Prod. 2009;30(3):333-7. [Link] [DOI:10.1016/j.indcrop.2009.06.001]
18. Adam K, Sivropoulou A, Kokkini S, Lanaras T, Arsenakis M. Antifungal activities of Origanum vulgare subsp. hirtum, Mentha spicata, Lavandula angustifolia, and Salvia fruticosa essential oils against human pathogenic fungi. J Agric Food Chem. 1998;46(5):1739-45. [Link] [DOI:10.1021/jf9708296]
19. Atai Z, Ansari M, Mousavi A, Mirzaei A. In-vitro study of antifungal effects of selected herbal extracts on standard and wild strains of Candida albicans. J Iran Dental Assoc. 2007;19(2):91-7. [Link]
20. Banaeian Bs, Mobini G, Rafeian Km, Rasti Bm, Sereshti M, Validi M. A comparative study of the effects of Clotrimazole and Salvia officinalis extract against vaginal Candida albicans isolated from women with candidacies vaginitis. Yafte. 2015;17(1):95-103. [Persian] [Link]
21. Sookto T, Srithavaj T, Thaweboon S, Thaweboon B, Shrestha B. In vitro effects of Salvia officinalis L. essential oil on Candida albicans. Asian Pac J Tropical Biomed. 2013;3(5):376-80. [Link] [DOI:10.1016/S2221-1691(13)60080-5]
22. Pozzatti P, Scheid LA, Spader TB, Atayde ML, Santurio JM, Alves SH. In vitro activity of essential oils extracted from plants used as spices against fluconazole-resistant and fluconazole-susceptible Candida spp. Can J Microbiol. 2008;54(11):950-6. [Link] [DOI:10.1139/W08-097]
23. Salari S, Bakhshi T, Sharififar F, Naseri A, Almani PGN. Evaluation of antifungal activity of standardized extract of Salvia rhytidea Benth.(Lamiaceae) against various Candida isolates. J Mycologie Med. 2016;26(4):323-30. [Link] [DOI:10.1016/j.mycmed.2016.06.003]
24. Lee I, Fishman NO, Zaoutis TE, Morales KH, Weiner MG, Synnestvedt M, et al. Risk factors for fluconazole-resistant Candida glabrata bloodstream infections. Arch Intern Med. 2009;169(4):379-83. [Link] [DOI:10.1001/archinte.169.4.379]
25. Won EJ, Choi MJ, Kim M-N, Yong D, Lee WG, Uh Y, et al. Fluconazole-resistant Candida glabrata bloodstream isolates, South Korea, 2008-2018. Emerg Infect Dis. 2021;27(3):779. [Link] [DOI:10.3201/eid2703.203482]
26. Zhumaliyeva G, Zhussupova A, Zhusupova GE, Błońska-Sikora E, Cerreto A, Omirbekova N, et al. Natural compounds of salvia l. genus and molecular mechanism of their biological activity. Biomedicines. 2023;11(12):3151. [Link] [DOI:10.3390/biomedicines11123151]
27. Sen Utsukarci B, Gürdal Abamor M, Bilgin M, Şatana D, Demirci B, Tan N, et al. Biological activities of various extracts from Salvia cassia Sam. ex Rech. f. and chemical composition of its most active extract. Rec Nat Prod. 2019;13(1)24-36. [Link] [DOI:10.25135/rnp.68.18.02.090]
28. Potente G, Bonvicini F, Gentilomi GA, Antognoni F. Anti-Candida activity of essential oils from Lamiaceae plants from the Mediterranean area and the Middle East. Antibiotics. 2020;9(7):395. [Link] [DOI:10.3390/antibiotics9070395]